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KMID : 0545120220320080967
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Journal of Microbiology and Biotechnology
2022 Volume.32 No. 8 p.967 ~ p.975
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Metagenome-Assembled Genomes of Komagataeibacter from Kombucha Exposed to Mars-Like Conditions Reveal the Secrets in Tolerating Extraterrestrial Stresses
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Lee Im-Chang
Podolich Olga
Brenig Bertram
Tiwari Sandeep
Azevedo Vasco
de Carvalho Daniel Santana
Uetanabaro Ana Paula Trovatti
Goes-Neto Aristoteles
Alzahrani Khalid J.
Kim Bong-Soo
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Abstract
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Kombucha mutualistic community (KMC) is composed by acetic acid bacteria and yeasts, producing fermented tea with health benefits. As part of the BIOlogy and Mars EXperiment (BIOMEX) project, the effect of Mars-like conditions on the KMC was analyzed. Here, we analyzed metagenome-assembled genomes (MAGs) of the Komagataeibacter, which is a predominant genus in KMC, to understand their roles in the KMC after exposure to Mars-like conditions (outside the International Space Station) based on functional genetic elements. We constructed three MAGs: K. hansenii, K. rhaeticus, and K. oboediens. Our results showed that (i) K. oboediens MAG functionally more complex than K. hansenii, (ii) K. hansenii is a keystone in KMCs with specific functional features to tolerate extreme stress, and (iii) genes related to the PPDK, betaine biosynthesis, polyamines biosynthesis, sulfate-sulfur assimilation pathway as well as type II toxin-antitoxin (TA) system, quorum sensing (QS) system, and cellulose production could play important roles in the resilience of KMC after exposure to Mars-like stress. Our findings show the potential mechanisms through which Komagataeibacter tolerates the extraterrestrial stress and will help to understand minimal microbial composition of KMC for space travelers.
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KEYWORD
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Kombucha, Komagataeibacter, metagenome assembled genome, mars-like condition, whole metagenome
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